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Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV

By Christian TangAudio PostMay 21, 2020

Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.

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Ultraviolet germicidal irradiation (UVGI)
is a disinfection method that uses
short-wavelength ultraviolet (UV-C) light
to kill or inactivate microorganisms by
destroying nucleic acids and disrupting
their DNA.

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